
1、個人簡歷
黃立平,女,博士,副研究員,豬消化道傳染病創(chuàng)新團隊,主要從事豬圓環(huán)病毒疫苗、診斷試劑盒分子生物學研究,主持國家自然基金青年項目和面上項目各1項、十三五和十四五重點研發(fā)項目子課題各1項、黑龍江省自然基金項目1項和哈爾濱市科技攻關項目1項,作為主要執(zhí)行人參與十一五國家科技支撐計劃項目、科技部農業(yè)科研成果轉化項目、公益性行業(yè)(農業(yè))科研專項經費項目、國家重點研發(fā)計劃課題和國家863計劃課題等5項。發(fā)表SCI文章40余篇,其中以第一作者或通訊作者發(fā)表SCI文章18篇;獲得發(fā)明專利3項;主持豬圓環(huán)病毒2型cELISA抗體檢測試劑盒的研發(fā)、參與豬圓環(huán)病毒2型滅活疫苗(LG株)的研發(fā),獲得新獸藥證書2項、中國農業(yè)科學院科學技術成果獎一等獎1項、中華農業(yè)科技三等獎1項和全國農牧漁業(yè)豐收獎二等獎1項。
2、聯(lián)系方式
(1)研究方向:動物疫苗與分子免疫學
(2)電話:15804519121 / 0451-51051883
(3)郵箱: huangliping@caas.cn
(4)通訊地址:黑龍江省哈爾濱市香坊區(qū)哈平路678號
3、教育經歷
2000.09-2004.06 西南農業(yè)大學 動物醫(yī)學專業(yè) 學士
2006.09-2009.06 中國農業(yè)科學院研究生院 預防獸醫(yī)學專業(yè) 碩士
1991.09-1996.06 中國農業(yè)科學院研究生院 預防獸醫(yī)學專業(yè) 博士
4、工作經歷
2017.01-至今 中國農業(yè)科學院哈爾濱獸醫(yī)研究所 副研究員
2012.11-2016.12 中國農業(yè)科學院哈爾濱獸醫(yī)研究所 助理研究員
2010.11-2012.11 比利時根特大學 聯(lián)合培養(yǎng)博士研究生
2004.07-2006.07 天津畜牧獸醫(yī)研究所 研究實習員
5、承擔項目
1. 十四五國家重點研發(fā)計劃,畜禽重要免疫抑制性病原的免疫逃逸機制-PCV2導致外周淋巴組織中B淋巴細胞缺失的分子機制(2022YFD1800304,2022/11-2025/12), 40萬, 主持;
2. 國家自然科學基金面上項目,基于冷凍電鏡的豬圓環(huán)病毒2型中和構象表位結構解析及其抗體介導的中和作用機制研究(31873012,2019/01-2022/12), 60萬元,主持;
3. 十三五國家重點研發(fā)計劃,羊重要病毒病多價多聯(lián)疫苗及工藝研究-藍舌病新型標記疫苗研究(2017YFD0500903,2017/07-2020/12), 24.26萬, 主持;
4. 國家自然科學基金青年基金,豬圓環(huán)病毒2型Cap和Rep蛋白相互作用及其在病毒組裝過程中的功能(31302110,2014.01-2016.12),22萬元,主持;
5. 黑龍江省自然科學基金面上項目,展示豬圓環(huán)病毒2 型廣譜構象中和表位疫苗毒株抗原性研究(C2015064,2015.07-2018.07),6萬元,主持;
6. 哈爾濱市應用技術研究和開發(fā)項目(青年后備人才A類),豬圓環(huán)病毒2型抗體和抗原檢測試劑盒的開發(fā)(2015RAQYJ066,2016.07-2017.12), 4萬元,主持。
6、代表論文與著作
1. Chong Yu, Mengxiang Cao, Yanwu Wei, Jianhang Liu, Hao Zhang, Changming Liu, Li Feng*, Liping Huang*. Evaluation of cross-immunity among major porcine circovirus type 2 genotypes by infection with PCV2b and PCV2d circulating strains, Veterinary Microbiology 283 (2023) 109796. (通訊作者)
2. Zhang, H., Shi, H., Wei, Y., Shi, D., Cao, M., Liu, J., Liu, J., Li, L., Liu, C., Feng, L., & Huang, L. (2023). Impact of porcine circovirus type 2 on porcine epidemic diarrhea virus replication in the IPI-FX cell line depends on the order of infection. Frontiers in microbiology, 14, 1162104. doi:10.3389/fmicb.2023.1162104. (通訊作者)
3. Chong Yu, Yanwu Wei, Hao Zhang, Jianhang Liu, Li Feng#, Changming Liu#, Liping Huang#. Rapid detection of porcine circovirus type 2 by a red latex microsphere immunochromatographic strip. Applied Microbiology and Biotechnology, 2022, doi.org/10.1007. (通訊作者)
4. Yiping Wang, Yanwu Wei, Hongli Wu, Li Feng#, Changmig Liu#, Liping Huang#. Specific inhibition of the interaction between pseudorabies virus DNA polymerase subunits UL30 and UL42 by a synthetic peptide. Veterinary Microbiology, 2022, 272,109517.(通訊作者)
5. LP Huang, ZZ Sun, DL Xia, YW Wei, EC Sun, HZ Zhu, HQ Bian, HL Wu, L Feng, JF Wang*, CM Liu*. Neutralization Mechanism of a Monoclonal Antibody Targeting a Porcine Circovirus Type 2 Cap Protein Conformational Epitope. Journal of Virology, 2020, DOI:10.1128/JVI.01836-19.
6. Huang L, Wei Y, Xia D, Liu D, Zhu H, Wu H, Feng L, Liu C. A broad spectrum monoclonal antibody against porcine circovirus type 2 for antigen and antibody detection. Appl Microbiol Biotechnol, 2019, doi:10.1007/s00253-019-09715-0.
7. Xia D#, Huang L#, Xie Y, Zhang X Wei Y, Liu D, Zhu H, Bian H, Feng L, Liu C. The prevalence and genetic diversity of porcine circovirus types 2 and 3 in Northeast China from 2015 to 2018. Archives of Virology, 2019, DOI: 10.1007/s00705-019-04336-4.(共同第一作者)
8. Liping Huang,Yiping Wang,Yanwu Wei,Dongjie Chen,Dan Liu,Wenjuan Du,Deli Xia,Hongli Wu,Li Feng,Changming Liu*, Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s, Applied Microbiology and biotechnology, 2016, 100(13):5933-5943.
9. Sun Encheng#, Huang Liping#, Xu Qingyuan, Wang Haixiu, Xue Xinmei, Lu Ping, Li Wenjing, Liu Wen, Bu Zhigao, Wu Donglai. Emergence of a Novel Bluetongue Virus Serotype, China, 2014. Transboundary and Emerging Diseases, 2016, Doi:10.1111/tbed.12560. (共同第一作者)
10. Liping Huang,Yiping Wang,Yanwu Wei,et al. Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s. Appl Microbiol Biotechnol,2016,100:5933–5943.
11.Sun JH, Huang LP, Wei YW, etal. Prevalence Of emerging porcine parvoviruses and their co-infections with porcine circovirus type 2 in China. Archives of Virology, 2015, 160(5): 1339-1344. (共同第1作者)
12. Jianhui Sun, Liping Huang, Yanwu Wei, et al. Identification of three PPV1 VP2 protein-specific B cell linear epitopes using monoclonal antibodies against baculovirus-expressed recombinant VP2 protein. Appl Microbiol Biotechnol, DOI 10.1007/s00253-015-6790-z. (共同第1作者)
13. Sun EC, Huang LP, Xu QY, et al. Emergence of a Novel Bluetongue Virus Serotype, China 2014. Transboundary and Emerging Diseases 2016, 63: 585–589 (共同第1作者)
14. Huang L.P., Lu Y.H., Wei Y.W., Guo L.J., Liu C.M. Identification of three new type-specific antigen epitopes in the capsid protein of porcine circovirus type 1. Archives of Virology 2012, DOI: 10.1007/s00705-012-1268-0.
15. Saha D.*, Huang L.P.*, Bussalleu E., Lefebvre D., Fort M., Doorsselaere J.V., Nauwynck H.J. Antigenic subtyping and epitopes’ competition analysis of porcine circovirus type 2 using monoclonal antibodies. Veterinary Microbiology 2012, 157:13–22. (共同第1作者)
16. Huang L.P., Lu Y.H., Wei Y.W., Guo L.J., Wu H.L., Zhang F.Y., Fu Y.J., Liu C.M. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research 2011, 161:115–23.
17. Huang L.P., Lu Y.H., Wei Y.W., Guo L.J., Liu C.M. Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2. BMC Microbiology 2011, 22:188.
18. Huang LP, Lu Y, Wei Y, Guo L, Liu C. Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Journal of Virol Methods 2011, 171: 26–33.
7、專利和標準
1.黃立平,危艷武,劉長明,豬圓環(huán)病毒2型捕獲ELISA檢測試劑盒,ZL201510030198.2
2.黃立平,劉長明,危艷武,豬圓環(huán)病毒2型競爭ELISA抗體檢測試劑盒,ZL201510409233.1
3.劉長明,黃立平,危艷武,陸月華,表達V5表位標簽的重組豬圓環(huán)病毒2型毒株及其應用, ZL20101059197.1
8、獲得獎勵
1.豬圓環(huán)病毒2型滅活疫苗產業(yè)化開發(fā)與推廣應用,全國農牧漁業(yè)豐收獎,二等獎,劉長明,危艷武,陸月華,張超范,黃立平,郭龍軍,2013年
2.豬圓環(huán)病毒疫苗及配套診斷技術的研究與產業(yè)化,中華農業(yè)科技獎,三等獎,劉長明,危艷武,陸月華,張超范,黃立平,郭龍軍,2013年
3.豬圓環(huán)病毒病防控關鍵技術創(chuàng)新與應用,中國農業(yè)科學院,科技進步獎,一等獎,劉長明,危艷武,陸月華,張超范,黃立平,郭龍軍,2013年
4. 劉長明,危艷武,陸月華,黃立平. 豬圓環(huán)病毒2型滅活疫苗(LG株),新獸藥注冊證書, 2010.9.19,農業(yè)部,(2010)新獸藥證字30號
5. 黃立平,劉長明,危艷武等. 豬圓環(huán)病毒2型cELISA抗體檢測試劑盒,新獸藥注冊證書, 2021.11.2,農業(yè)農村部,(2021)新獸藥證字66號