1、個人簡歷
李剛，男，副研究員，碩士研究生導(dǎo)師，動物細菌病創(chuàng)新團隊骨干成員，主要從事豬呼吸道細菌病致病機制及新型疫苗研究，先后主持和參與國家自然科學(xué)基金等項目。在Journal of Bacteriology、Journal of Proteomics等國內(nèi)外重要學(xué)術(shù)刊物發(fā)表論文20多篇，獲得省市級獎勵2項，獲得國家發(fā)明專利10余項。
2、聯(lián)系方式
研究方向：獸醫(yī)微生物及其分子生物學(xué)
電話：15245153569
郵箱：ligang@caas.cn
通訊地址：黑龍江省哈爾濱市香坊區(qū)哈平路678號
3、教育經(jīng)歷
1998.09-2002.07 東北農(nóng)業(yè)大學(xué) 植物保護 學(xué)士
2002.09-2005.07 東北農(nóng)業(yè)大學(xué) 植物病理 碩士
2005.09-2010.04 中國科學(xué)院微生物研究所 微生物學(xué) 博士
4、工作經(jīng)歷
2010.07-2011.01 中國農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 研究實習(xí)員
2011.01-2018.12 中國農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 助理研究員
2018.05-2019.05 中國生物技術(shù)發(fā)展中心戰(zhàn)略與政策處 借調(diào)
2019.01-至今 中國農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 副研究員
5、承擔(dān)項目
國家自然科學(xué)基金面上項目：(p)ppGpp靶標(biāo)蛋白MnmE調(diào)控胸膜肺炎放線桿菌致病力的機制研究（32373013，2024.01-2027.12），50萬元，主持；
國家自然科學(xué)基金面上項目：(p)ppGpp誘導(dǎo)胸膜肺炎放線桿菌形成持留菌的分子機制研究（31672575，2017.01-2020.12），62萬元，主持；
國家自然科學(xué)基金青年項目：氨基酸饑餓狀態(tài)下(p)ppGpp調(diào)控胸膜肺炎放線桿菌致病性研究（31302091，2014.01-2016.12），25萬元，主持；
黑龍江省自然科學(xué)基金面上項目：胸膜肺炎放線桿菌持留菌形成的分子機制研究（C2017078，2017.01-2020.12），6萬元，主持；
國家自然科學(xué)基金面上項目：豬鏈球菌的eSTK/eSTP系統(tǒng)對反應(yīng)調(diào)節(jié)因子CovR的調(diào)控及其機制研究（31772757，2018.01 -2021.12），62萬，參與；
國家自然科學(xué)基金青年項目：SK2b型A族鏈球菌激活纖溶酶原的分子機制的研究（31500112，2016.01-2018.12），19萬，參與;
中央級公益性科研院所基本科研業(yè)務(wù)費項目：胸膜肺炎放線桿菌relA突變株的構(gòu)建及其生物學(xué)特性研究（SKLVBP201418，2014.01-2014.12），10萬元，主持；
中央級公益性科研院所基本科研業(yè)務(wù)費項目：副豬嗜血桿菌細胞致死膨脹毒素CDT的致病機理研究（0302012014，2012.01-2013.12），10萬元，主持；
中央級公益性科研院所基本科研業(yè)務(wù)費項目：鴨疫里默氏桿菌毒力因子的鑒定及功能研究（SKLVBP201135，2011.01-2011.12），10萬元，主持。
6、代表論文與著作
Lu Wang, Jing Sun, Jiyu Zhao, JieYu Bai，Yueling Zhang, Yao zhu, Wanjiang Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Gang Li*. A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2.Talanta. 267 (2024) 125202
Li Guo, Gulimire Niyazi, Lingling Huang, Pengyu Zhao, Zifeng Wang, Jiao Lin, Tianhui Li, Gang Li, Liqiang Song, Dingxin Liu, Xiaohua Wang, Mingzhe Rong. Inactivation effects of the mist nebulized with plasma-activated air on Pseudomonas aeruginosa through the simulated respiratory tract. Plasma Processes And Polymers. 2023, 20: e2200204
Tian Luan, Lu Wang, Jiyu Zhao, Hui Luan, Yueling Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Wanjiang Zhang and Gang Li*. A CRISPR/Cas12a-assisted rapid detection platform by biosensing the apxIVA of Actinobacillus pleuropneumoniae. Frontiers in Microbiology. 2022,13: 928307
Guangjuan Gao, Dong Wei, Gang Li, Ping Chen, Liujun Wu, Siguo Liu, Yueling Zhang. Highly Effective Markerless Genetic Manipulation of Streptococcus suis Using a Mutated PheS-Based Counterselectable Marker. Frontiers in Microbiology. 2022,13: 947821
Yue Jia, Changqi Hao, Qin Yang, Wanjiang Zhang, Gang Li, Siguo Liu, Xin Hua. Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action. Research in Veterinary Science. 2021;138:62-68
Zifeng Wang, Yu Qi, Li Guo,Lingling Huang, Zhiqian Yao, Lu Yang, Gang Li, Jinkun Chen, Jinwei Yan, Gulimire Niyazi, Linbo Liu, Fugao Zhang, Dingxin Liu. The bactericidal effects of plasma-activated saline prepared by the combination of surface discharge plasma and plasma jet. Journal of Physics D: Applied Physics. 54 (2021),385202.
Gang Li, Qian Zhao, Tian Luan, Yangbo Hu, Yueling Zhang, Ting Li, Chunlai Wang, Fang Xie, Wanjiang Zhang, Paul R. Langford, Siguo Liu*. Basal Level Effects of (p)ppGpp in the Absence of Branched Chain Amino Acids in Actinobacillus pleuropneumoniae. Journal of Bacteriology. 2020, 202:e00640-19
Ping Chen, Ran Liu, Mengmeng Huang, Jinlu Zhu, Dong Wei, Francis J Castellino, Guanghui Dang, Fang Xie, Gang Li, Ziyin Cui, Siguo Liu, Yueling Zhang. A unique combination of glycoside hydrolases in Streptococcus suis specifically and sequentially acts on host-derived αGal-epitope glycans. Journal of Biological Chemistry. 2020, 295(31):10638-10652.
Gang Li, Fang Xie, Jianjun Li, Jiao Liu, Dapeng Li, Yanhe Zhang, Paul R. Langford, Yanwen Li, Siguo Liu, Chunlai Wang*. Identification of novel vaccine candidates of Haemophilus parasuis serovar 5 by immunoproteomics approach. Journal of Proteomics. 2017, 163:111-117.
Gang Li, Hui Niu, Yanhe Zhang, Yanling Li, Fang Xie, Paul R. Langford, Siguo Liu, Chunlai Wang*. Haemophilus parasuis Cytolethal Distending Toxin Induces Cell Cycle Arrest and p53-dependent Apoptosis. PLoS One. 2017, 12(5):e0177199.
Fang Xie?, Gang Li?, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang*. Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8?clpP/apxIIC confers protection against homologous or heterologous strain challenge. BMC Veterinary Research. 2017, 13(1):14.
Fang Xie, Gang Li, Yalei Wang, Yanhe Zhang, Long Zhou, Chengcheng Wang, Shuanghong Liu, Siguo Liu, Chunlai Wang*. Pyridoxal phosphate synthases PdxS/PdxT are required for Actinobacillus pleuropneumoniae viability, stress tolerance and virulence, PLoS One, 2017, 12(4): e0176374.
Fang Xie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R. Langford, Chunlai Wang. The SapA protein is involved in resistance to antimicrobial peptide PR-39 and virulence of Actinobacillus pleuropneumoniae, Frontiers in Microbiology, 2017, 8:811.
Yanhe Zhang, Gang Li, Fang Xie, Siguo Liu, Chunlai Wang*. Evaluation of glutathione-binding protein A of Haemophilus parasuis as a vaccine candidate in a mouse model, J Vet Med Sci, 2017, 79(1):184-187
Fang Xie, Gang Li, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang*. The Lon protease homologue LonA, not LonC, contributes to the stress tolerance and biofilm formation of Actinobacillus pleuropneumoniae, Microb Pathog, 2016, 93:38-43.
Fang Xie, Gang Li, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang*. Outer membrane lipoprotein VacJ is required for the membrane integrity and virulence of Actinobacillus pleuropneumoniae, Vet Microbiol, 2016, 183: 1-8.
Gang Li, Fang Xie, Yanhe Zhang, Janine T. Bossé2, Paul R. Langford, Chunlai Wang*. Role of (p)ppGpp in viability and biofilm formation of Actinobacillus pleuropneumoniae. PLoS One. 2015, (10): e0141501.
Mingming Hu, Yanhe Zhang, Fang Xie, Gang Li, Jianjun Li, Wei Si, Siguo Liu, Shouping Hu, Zhuo Zhang, Nan Shen, Chunlai Wang. Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge. Clinical and Vaccine Immunology. 2013, 20(6):795-802.
Fang Xie, Yanhe Zhang, Gang Li, Long Zhou, Siguo Liu, Chunlai Wang*. The ClpP Protease Is Required for the Stress Tolerance and Biofilm Formation in Actinobacillus pleuropneumoniae. PLoS One. 2013;8(1):e53600.
Gang Li, Fang Xie, Yanhe Zhang, and Chunlai Wang*. Draft genome sequence of Actinobacillus pleuropneumoniae serotype 7 strain S-8, Journal of Bacteriology. 2012, 194(23):6606-6607.
Ming Liu, Chunguo Liu, Gang Li, Xiaojun Li, Xiuchen Yin, Yuhuan Chen, and Yun Zhang. Complete Genomic Sequence of Duck Flavivirus from China. Journal of Virology. 2012. 86(6):3398-9.
Ming Liu, Shaoying Chen,Yuhuan Chen, Chunguo Liu, Shilong Chen, Xiuchen Yin, Gang Li, and Yun Zhang. Adapted Tembusu-Like Virus in Chickens and Geese in China. Journal of Clinical Microbiology. 2012. 50(8):2807-9.
Gang Li, Yajian Song, Yanfen Xue, Lang Rao, Chen Zhou, Quanhui Wang, Yanhe Ma . Comparative proteome analysis of alkaliphilic Bacillus sp. N16-5 grown on different carbon sources. Science China Life Science. 2011;54(1):90-100.
Gang Li, Lang Rao, Yanfen Xue, Chen Zhou, Yun Zhang, Yanhe Ma. Cloning, expression, and characterization of a highly active alkaline pectate lyase from alkaliphilic Bacillus sp. N16-5. Journal of Microbiological Biotechnology. 2010, 20(4):670-7.
趙濟鈺, 王璐，孫婧, 王春來，李剛*，劉思國*. 胸膜肺炎放線桿菌MnmE 基因突變株的構(gòu)建及其生物學(xué)特性的研究，2023, 45(3)225-231.
欒天, 龔俊, 欒慧, 劉文宇, 楊親, ?，?/span>, 王春來, 劉思國, 張萬江*, 李剛*. 利用CRISPR/Cas12a 技術(shù)快速檢測胸膜肺炎放線桿菌方法的建立,2021, 43(8)843-847.
7、專利和標(biāo)準(zhǔn)
李剛，張躍靈，王春來，劉思國?；?/span>CRISPR-Cas12ａ的胸膜肺炎放線桿菌快速可視化檢測方法，申請?zhí)枺?/span>202011643292.2
李剛，張躍靈，王春來，劉思國。一種基于CRISPR-Cas12a的豬鏈球菌快速可視化RPA檢測試劑盒及其應(yīng)用，申請?zhí)枺?/span>202110863649.6
李剛，張躍靈，王春來，張萬江，劉思國。用于豬鏈球菌2型和1/2型、1型和14型快速分型的試劑盒及其應(yīng)用, 申請?zhí)枺?/span>202311199564.8
李剛，王春來，張艷禾，謝芳。一種副豬嗜血桿菌免疫保護性抗原HbpA ZL , 201310268864.7
王春來，李剛，張艷禾，謝芳。一種副豬嗜血桿菌病亞單位疫苗組合物及應(yīng)用ZL 201310406551.3
王春來，李剛，張艷禾，謝芳。一種副豬嗜血桿菌免疫保護性抗原OppA2 ZL 201310268810.0
王春來，李剛，張艷禾，謝芳。一種副豬嗜血桿菌免疫保護性抗原AfuA ZL 201310268347.X
王春來，李剛，張艷禾，謝芳。一種副豬嗜血桿菌免疫保護性抗原OppA ZL 201310268863.2
王春來，張艷禾，李剛，謝芳。一種制備副豬嗜血桿菌病菌影疫苗的方法及其產(chǎn)品和應(yīng)用ZL 201310409237.0
王春來，謝芳，李剛，張艷禾。一種不含抗性標(biāo)記的豬胸膜肺炎弱毒活疫苗ZL 201310469680.7
謝芳，王春來，張艷禾，李剛。一種不含抗性標(biāo)記的豬胸膜肺炎放線桿菌ClpP蛋白酶基因缺失株、其構(gòu)建方法及應(yīng)用ZL 201110336861.3
8、獲得獎勵
豬傳染性胸膜肺炎菌影疫苗的研制及其應(yīng)用，黑龍江省科技進步三等獎（王春來，李剛，謝芳，張艷禾，李艷玲，周瀧），2017年。
豬傳染性胸膜肺炎菌影疫苗的研制及其應(yīng)用，哈爾濱市科技進步二等獎（王春來，李剛，謝芳，張艷禾，李艷玲，周瀧），2016年。
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