走進(jìn)哈獸研
綜合新聞
科研進(jìn)展
科研成果
人才招聘
辦公系統(tǒng)
掃一掃 關(guān)注我
1、個(gè)人簡(jiǎn)歷
劉思國(guó)，男，博士，研究員，博士生導(dǎo)師。中國(guó)農(nóng)業(yè)科學(xué)院三級(jí)崗位科學(xué)家，中國(guó)防癆協(xié)會(huì)人獸共患結(jié)核病專業(yè)分會(huì)副主任委員、農(nóng)業(yè)部第七屆獸藥評(píng)審專家。研究方向?yàn)閯?dòng)物結(jié)核病和副結(jié)核病致病機(jī)理及防控技術(shù)研究。承擔(dān)了973項(xiàng)目、863項(xiàng)目、重點(diǎn)研發(fā)計(jì)劃和國(guó)家自然科學(xué)基金項(xiàng)目等20余項(xiàng)課題。發(fā)表SCI收錄文章100余篇，其中以第1或通訊作者發(fā)表76篇。著作8部，獲省部級(jí)獎(jiǎng)6項(xiàng)，獲發(fā)明專利24項(xiàng)。
2、聯(lián)系方式
研究方向：動(dòng)物傳染病病原學(xué)與流行病學(xué)
電話：0451-51051737
郵箱：liusiguo@caas.cn
通訊地址：黑龍江省哈爾濱市香坊區(qū)哈平路678號(hào)，郵編150069。
3、教育經(jīng)歷
1987.09-1991.06 吉林農(nóng)業(yè)大學(xué) 獸醫(yī)公共衛(wèi)生專業(yè) 學(xué)士
1991.09-1994.06 吉林農(nóng)業(yè)大學(xué) 獸醫(yī)微生物和免疫學(xué)專業(yè) 碩士
1996.09-1999.06 東北農(nóng)業(yè)大學(xué) 獸醫(yī)病理學(xué)專業(yè) 博士
4、工作經(jīng)歷
2009.1-至今 中國(guó)農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 主任/研究員
2001.12-2008.12 中國(guó)農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 副研究員
1994.07-2001.12 中國(guó)農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所 助理研究員
1999.12-2001.12 中國(guó)人民解放軍軍需大學(xué)軍事獸醫(yī)研究所 博士后
5、承擔(dān)項(xiàng)目
1. 十四五國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目：胞內(nèi)菌入侵與持續(xù)感染機(jī)制研究，2021.06.01-2025.12.31，172萬元，劉思國(guó)主持（子課題）；
2. 國(guó)家自然科學(xué)基金面上項(xiàng)目：結(jié)核分枝桿菌Rv2569c半胱氨酸蛋白酶介導(dǎo)的Caspase非依賴性巨噬細(xì)胞凋亡的機(jī)制研究（32273005, 2023-2026）,54萬元；
3. 國(guó)家自然科學(xué)基金面上項(xiàng)目：結(jié)核分枝桿菌胞外鞘磷脂酶Rv0888誘導(dǎo)肺臟中性粒細(xì)胞胞外誘捕網(wǎng)形成繼而促進(jìn)炎癥反應(yīng)的機(jī)制研究（31772767，2018.01-2021.12），58萬元；
4. 農(nóng)業(yè)部行業(yè)專項(xiàng)：東北地區(qū)畜禽沙門菌耐藥性分析及防控技術(shù)研究（201403054，2014-2018），110萬元；
5. 農(nóng)業(yè)部行業(yè)專項(xiàng)，豬傳染性胸膜肺炎放線桿菌的病原分離鑒定與流行病學(xué)調(diào)查研究（201303034，2013-2017），123萬元；
6、代表論文與著作
1. Zang X, Zhang J, Jiang Y, Feng T, Cui Y, Wang H, Cui Z, Dang G*, Liu S*. Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin. PLoS Pathog. 2024 May 9;20(5):e1012214.
2. Zhang J, Cui Y, Zang X, Feng T, Chen F, Wang H, Dang G, Liu S. PE12 interaction with TLR4 promotes intracellular survival of Mycobacterium tuberculosis by suppressing inflammatory response. Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127547.
3. Tang Yang#, Cui Y#, Jiang Yan, Shao M, Zang Xin, Dang G*, Liu S*. Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice. J Integr Agric. 2023 22(12):3755-3768.
4. Yang Qin, Zhu Yao, Schwarz Stefan, Wang Lingli, Yang Wenlin, Liu Siguo*, Zhang Wanjiang*. Integrative and conjugative elements in streptococci can act as vectors for plasmids and translocatable units integrated via IS1216E. International Journal of Antimicrobial Agents. 2023 Mar 16:106793.
5. Shao M, Cui N, Tang Y, Chen F, Cui Y, Dang G*, Liu S*. A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in mice. NPJ Vaccines. 2023 May 20;8(1):72.
6. Yue Jia, Juzhao Liu, Qin Yang, Wanjiang Zhang, Thomas Efferth, Siguo Liu*, Xin Hua*. Cajanin stilbene acid：A direct inhibitor of MCR-1 that restores the efficacy of polymyxin B against resistant Gram-negative bacteria. Phytomedicine. 2023,114:154803.
7. Cui Y, Tang Y, Shao M, Zang X, Jiang Y, Cui Z, Dang G*, Liu S*. Mycobacterium tuberculosis protease Rv3090 is associated with late cell apoptosis and participates in organ injuries and mycobacterial dissemination in mice. Microb Pathog. 2022 Dec;173(Pt B):105880.
8. Zang X#, Dang G#, Cai Z, Shao M, Tang Y, Cao J, Cui Z, Liu S*. Extracellular DNase MAP3916c attacks the neutrophil extracellular traps and is needed for Mycobacterium avium subsp. paratuberculosis virulence. Vet Microbiol. 2022 Oct;273:109529.
9. Cui Y, Dang G, Wang H, Tang Y, Lv M, Zang X, Cai Z, Cui Z, Cao J, Liu S*, Song N*. DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin. DNA Cell Biol. 2022 Dec;41(12):1063-1074.
10. Cui Z, Dang G, Song N, Cui Y, Li Z, Zang X, Liu H, Wang Z, Liu S*. Rv3091, an extracellular patatin-like phospholipase in Mycobacterium tuberculosis, prolongs intracellular survival of recombinan Mycolicibacterium smegmatis by mediating phagosomal escape. Front Microbiol, 2020, 11:2204.
11. Xie F, Zan Y, Zhang X, Zhang H, Jin M, Zhang W, Zhang Y, Liu S. Differential abilities of mammalian cathelicidins to inhibit bacterial biofilm formation and promote multifaceted immune functions of neutrophils. Int J Mol Sci, 2020, 21(5):1871.
12. Song N, Zhu Y, Cui Y, Lv M, Tang Y, Cui Z, Dang G, Zheng H, Liu S. Vitamin B and vitamin C affect DNA methylation and amino acid metabolism in Mycobacterium bovis BCG. Front Microbiol, 2020, 11:812.
13. Zhu Y, Zhang W, Wang C, Liu W, Yang Q, Luan T, Wang L, Schwarz S, Liu S. Identification of a novel optrA-harbouring transposon, Tn6823, in Staphylococcus aureus. J Antimicrob Chemother, 2020, 75(11):3395-3397.
14. Liu H, Dang G, Zang X, Cai Z, Cui Z, Song N, Liu S. Characterization and pathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp. paratuberculosis. Microb Pathog, 2020, 142:104055.
15. Li G, Zhao Q, Luan T, Hu Y, Zhang Y, Li T, Wang C, Xie F, Zhang W, Langford PR, Liu S. Basal-level effects of (p)ppGpp in the absence of branched-chain amino acids in Actinobacillus pleuropneumoniae. J Bacteriol, 2020, 202(8):e00640-19.
16. Xie F, Zan Y, Zhang Y, Zheng N, Yan Q, Zhang W, Zhang H, Jin M, Chen F, Zhang X, Liu S. The cysteine protease ApdS from Streptococcus suis promotes evasion of innate immune defenses by cleaving the antimicrobial peptide cathelicidin LL-37. J Biol Chem, 2019, 294(47):17962-17977.
17. Wang C, Li P, Yan Q, Chen L, Li T, Zhang W, Li H, Chen C, Han X, Zhang S, Xu M, Li B, Zhang X, Ni H, Ma Y, Dong B, Li S, Liu S. Characterization of the pig gut microbiome and antibiotic resistome in industrialized feedlots in China. mSystems, 2019, 4(6): e00206-19.
18. Zhang W, Zhu Y, Wang C, Liu W, Li R, Chen F, Luan T, Zhang Y, Schwarz S, Liu S. Characterization of a multidrug-resistant porcine Klebsiella pneumoniae sequence type 11 strain coharboring blaKPC-2 and fosA3 on two novel hybrid plasmids. mSphere, 2019, 4(5):e00590-19.
19. Hua X, Jia Y, Yang Q, Zhang W, Dong Z, Liu S. Transcriptional analysis of the effects of gambogic acid and neogambogic acid on methicillin-resistant Staphylococcus aureus. Front Pharmacol, 2019, 10:986.
20. Chen F, Zhang W, Schwarz S, Zhu Y, Li R, Hua X, Liu S. Genetic characterization of an MDR/virulence genomic element carrying two T6SS gene clusters in a clinical Klebsiella pneumoniae isolate of swine origin. J Antimicrob Chemother, 2019, 74(6):1539-1544.
21. Zhu Y, Zhang W, Schwarz S, Wang C, Liu W, Chen F, Luan T, Liu S. Characterization of a blaIMP-4-carrying plasmid from Enterobacter cloacae of swine origin. J Antimicrob Chemother, 2019, 74(7):1799-180.
22. Li H, Dang G, Liu H, Wang Z, Cui Z, Song N, Chen L, Liu S. Characterization of a novel Mycobacterium tuberculosis serine protease (Rv3194c) activity and pathogenicity. Tuberculosis, 2019, 119:101880.
23. Song N, Li Z, Cui Z, Chen L, Cui Y, Dang G, Li Z, Li H, Liu S. The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1. BMC Microbiol, 2019, 19(1):104.
24. Dang G, Cui Y, Wang L, Li T, Cui Z, Song N, Chen L, Pang H, Liu S. Extracellular sphingomyelinase Rv0888 of Mycobacterium tuberculosis contributes to pathological lung injury of Mycobacterium smegmatis in mice via inducing formation of neutrophil extracellular traps. Front Immunol, 2018, 9:677.
25. Hua X, Yang Q, Zhang W, Dong Z, Yu S, Schwarz S, Liu S. Antibacterial activity and mechanism of action of aspidinol against multi-drug-resistant methicillin-resistant Staphylococcus aureus. Front Pharmacol, 2018, 9:619.
26. Wei Si, Shenye Yu, Henggui Liu, Chunlai Wang, Liping Chen, Gaoling Wang, Jianan Wu, Siguo Liu*. A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine. Vet Microbiol, 2017, 203:189-195.
27. Xiu-Mei Wang, Zhimin Dong, Stefan Schwarz, Zhu Yao, Xin Hua, Yanhe Zhang, Siguo Liu* and Wan-Jiang Zhang*. Plasmids of diverse Inc groups disseminate the fosfomycin resistance gene fosA3 among Escherichia coli isolates from pigs, chickens, and dairy cows in northeast China. Antimicrob Agents Chemother, 2017, 61(9): e00859.
28. Guanghui Dang, Jun Cao, Yingying Cui, Ningning Song, Liping Chen*, Hai Pang*, Siguo Liu*. Characterization of Rv0888, a novel extracellular nuclease from Mycobacterium tuberculosis. Sci Rep, 2016, 6:10933.
29. Wan-Jiang Zhang, Xiu-Mei Wang, Lei Dai, Xin Hua, Zhimin Dong, Stefan Schwarz, and Siguo Liu*. Novel conjugative plasmid from Escherichia coli of swine origin that coharbors the multiresistance gene cfr and the extended-spectrum-β-lactamase gene blaCTX-M-14b. Antimicrob Agents Chemother. 2015, 59(2):1337-1340.
30. Hongbin Si, Wan-Jiang Zhang, Shengbo Chu, Xiu-Mei Wang, Lei Dai, Xin Hua, Zhimin Dong, Stefan Schwarz, and Siguo Liu*, Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin. Antimicrob Agents Chemother, 2015, 59(11), 7113-7116.
31. Jun Cao, Guanghui Dang, Huafang Li, Tiantian Li, Zhiguo Yue, Na Li, Yajun Liu, Siguo Liu*, Liping Chen*. Identification and characterization of lipase activity and immunogenicity of LipL from Mycobacterium tuberculosis. PLoS One, 2015, 10(9): e0138151.
32. Wan-Jiang Zhang, Xing-Ran Xu, Stefan Schwarz, Xiu-Mei Wang, Lei Dai, Hua-Jun Zheng and Siguo Liu*. Chracterization of the IncA/C plasmid pSCEC2 harboring the multiresistance cfr gene from Escherichia coli of swine origin. J Antimicrob Chemother, 2014, 69(2):385-389.
33. XiuMei Wang, Xing Feng Lu, Lu Yin, HuiFang Liu, WanJiang Zhang, Wei Si, ShenYe Yu, Meili Shao*, Siguo Liu*. Occurrence and antimicrobial susceptibility of Listeria Monocytogens isolate from retail raw foods. Food Control, 2013, 32: 153-158.
34. Xianglian Yuan*, Liping Chen*, Jun Cao, Qiulong Yan, Yinbin Liu, Guanghui Dang, Shenye Yu, Wei Si, Xiumei Wang, Hai Pang*, Siguo Liu*. Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Tuberculosis, 2013, 93:396-300.
35. Wan-Jiang Zhang, Zongji Lu, Stefan Schwarz, Rong min Zhang, ShenYe Yu, Wei Si, LiPing Chen, Siguo Liu*. Complete sequence of the blaNDM-1-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin. 2013, J Antimicrob Chemother, 2013, 68(7): 1681-1682.
36. Wang XM, Zhang WJ, Schwarz S, Yu SY, Liu H, Si W, Zhang RM, Siguo Liu*. Methicillin-resistant Staphylococcus aureus ST9 from a case of bovine mastitis carries the genes cfr and erm(A) on a small plasmid. J Antimicrob Chemother, 2012, 67(5):1287-1289.
37. Wei Peng§, Lu Yin§, Wei Si§, Huifang Liu, Shenye Yu, Siguo Liu*, Chunlai Wang, Yuehong Chang, Zhuo Zhang, Shouping Hu, Yanfen Du. Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. Immunobiology, 2011, 216:558-565.
38. Jiubiao Guo, Lipeng Xu, Xiangdong Zeng, shentao Li, Xianggang Kong, Siguo Liu* and Hai Pang*. Crystal structure of a novel esterase Rv0045c from Mycobacterium tuberculosis. PLoS One, 2011, 6(5) e20506.
39. JiubiaoGuo, Xiangdong Zheng, Lipeng Xu, Zhongyuan Liu, Kehui Xu, Shentao Li, Tingyi Wen, Siguo Liu* and Hai Pang*. Characterization of a novel esterase Rv0045c from Mycobacterium tuberculosis, PLoS One, 2010, 5(10): e13143.
40. Si-Guo Liu, She-Ping Guo, Chun-Lai Wang, Mei-Li Shao, Xiu-Hua Zhang, Yang-Guo, Qiang-Gong. A novel fusion protein based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis. Tuberculosis, 2007, 3: 212-217.
7、專利和標(biāo)準(zhǔn)
1. 黨光輝，劉思國(guó)，蔡珠明，崔子寅. 一種同時(shí)檢測(cè)三種分枝桿菌的多重TaqMan熒光定量PCR試劑盒及其應(yīng)用. 專利號(hào)：ZL202110731289.4
2. 劉思國(guó)，黨光輝，陳利蘋，李田田. 一種牛副結(jié)核間接ELISA檢測(cè)試劑盒及其應(yīng)用. 專利號(hào)：ZL202310215284.5
3. 于申業(yè)，劉思國(guó). 抗大腸桿菌感染的治療性單克隆抗體、產(chǎn)生該單克隆抗體的雜交瘤細(xì)胞株及其用途（ZL201410412146.7），2017年；
4. 于申業(yè)，劉思國(guó). 抗phiX174噬菌體裂解蛋白E的單克隆抗體、產(chǎn)生該抗體的雜交瘤細(xì)胞株及其表位（ZL201210001050.2），2013年；
5. 劉思國(guó)，黎觀紅，賈永杰，洪智敏，司微，于申業(yè). 一種乳酸桿菌細(xì)胞壁完整肽聚糖及其在提高IEC抗菌肽表達(dá)水平中的應(yīng)用（ZL201210122033.4），2015年；
6. 劉思國(guó)，王華南，于申業(yè)，朱婷，于秀婷，劉慧芳，王秀梅，司微. 抗結(jié)核分枝桿菌單克隆抗體及其識(shí)別的抗原表位和用途（ZL201110269165.5），2013年；
7. 劉思國(guó)，劉慧芳，王秀梅，于申業(yè)，王牟平，司微，楊志. 減毒沙門氏菌及其應(yīng)用（ZL201110061628.9），2012年；
8. 劉思國(guó)，常月紅，彭偉，劉慧芳，王春來，司微. 重排噬菌體溶菌基因及其打孔載體和在制備疫苗中的應(yīng)用（ZL200910118379.5），2011年；
9. 劉思國(guó)，彭偉，常月紅，劉慧芳，王春來，司微. 增強(qiáng)噬菌體溶菌基因打孔效率的DNA序列及其應(yīng)用（ZL200910118380.8），2011年；
10. 劉思國(guó)，郭設(shè)平，王春來，張秀華，郭洋，邵美麗.一種診斷牛結(jié)核病的重組抗原蛋白及其制備方法（ZL200510005002.0），2008年；
8、獲得獎(jiǎng)勵(lì)
1. 人獸共患性結(jié)核病新型診斷技術(shù)及其在牛結(jié)核控制和凈化中的應(yīng)用，湖北省技術(shù)發(fā)明二等獎(jiǎng)，郭愛珍、陳穎鈺、劉思國(guó)、趙洪進(jìn)、但漢并、李雪平（華中農(nóng)業(yè)大學(xué)、中國(guó)農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所、上海市動(dòng)物疫病預(yù)防控制中心、武漢科前生物股份有限公司、廣州悅洋生物技術(shù)有限公司），2020年；
2. 人畜間結(jié)核病流行病學(xué)的調(diào)查研究及推廣應(yīng)用，中華人民共和國(guó)農(nóng)業(yè)部農(nóng)牧漁業(yè)豐收獎(jiǎng)三等獎(jiǎng)，李愛巧，呼西旦.阿巴拜克力，劉思國(guó)，楊啟元，施遠(yuǎn)翔，王六合，陳利蘋，李君蓮， 陳飛，帕提瑪?努合曼，程維疆，何生，郭亞軍，王愛菊，方新元，張成成，郭新鋼，劉軍成，周棕長(zhǎng)，王杏芹，賽力克江，韓義忠，舒峰，彭華剛，羅文毅，2016年；
3. 牛結(jié)核病病原生態(tài)學(xué)和流行病學(xué)研究，黑龍江省自然科學(xué)三等獎(jiǎng)，劉思國(guó)，司微，于申業(yè)，王牟平，陳利蘋，2014年；
4. 牛結(jié)核病病原生態(tài)學(xué)和流行病學(xué)研究，中國(guó)農(nóng)業(yè)科學(xué)院自然科學(xué)二等獎(jiǎng)，劉思國(guó)，劉慧芳，司微，王春來，于申業(yè)，王牟平，2012年；
掃一掃 關(guān)注我
網(wǎng)站首頁
聯(lián)系我們